Journal: Animal : an international journal of animal bioscience

Article Title: The GH/IGF1 axis in the kidney of young goats fed a protein-reduced diet.

doi: 10.1016/j.animal.2023.100897

Figure Lengend Snippet: Fig. 2. Western blot analysis and semi-quantification of (A) growth hormone receptor (GHR) protein, (B) IGF1 (IGF1R) protein, (C) insulin receptor (INSR) protein, (D) Janus kinase 2 (JAK2) protein, (E) suppressor of cytokine signalling 2 (SOCS2) protein, (F) suppressor of cytokine signalling 3 (SOCS3) protein, (G) signal transducers and activators of transcription 1 (STAT1) protein, (H) signal transducers and activators of transcription 3 (STAT3) protein, and (I) signal transducers and activators of transcription 5B (STAT5B) protein expression in the renal cortex of young goats fed a control diet (N + ) or a reduced-protein diet (N-). M, molecular weight marker. Values are means with their SEM represented by vertical bars. *Significant differences between the two groups were revealed by an unpaired Student’s t-test (P < 0.05); **P < 0.01; ****P < 0.001.

Article Snippet: GHR, JAK2, SOCS2, SOCS3, STAT1, STAT3, STAT5B, IGF1R and INSR protein were detected by treating the membranes overnight at 4 C with anti-GHR antibody (Santa Cruz Biotechnology Inc., Heidelberg, Germany), anti-JAK2 antibody (Cell Signalling), anti-SOCS2 antibody (Cell Signalling), anti-SOCS3 antibody (Cell Signalling), anti-STAT1 antibody (Cell Signalling), antiSTAT3 antibody (Cell Signalling), anti-STAT5B antibody (GeneTex Inc., Irvine, CA, USA), anti-IGF1R antibody (Cell Signalling) or with anti-INSR antibody (Santa Cruz Biotechnology).

Techniques: Western Blot, Expressing, Control, Molecular Weight, Marker